Oral Presentation Australasian Society for Immunology Annual Scientific Meeting 2014

Surface profiles of live colorectal cancer cells and tumour infiltrating lymphocytes from surgical samples correspond to prognostic categories (#146)

Richard I Christopherson 1 , Jerry Zhou 1 , Larissa Belov 1 , Pierre Chapuis 2 , Charles Chan 3 , Nicola Armstrong 4 , Kimberley L Kaufman 1 , Michael J Solomon 5 , Stephen J Clarke 6
  1. University of Sydney, Sydney, NSW, Australia
  2. Department of Colorectal Surgery, Concord Hospital, Concord West, NSW, Australia
  3. Department of Anatomical Pathology, Concord Hospital, Concord West, NSW, Australia
  4. School of Mathematics and Statistics, University of Sydney, Sydney, NSW, Australia
  5. Discipline of Surgery, Royal Prince Alfred Hospital, Camperdown, NSW, Australia
  6. Bill Walsh Cancer Research Laboratories, Royal North Shore Hospital, St Leonards, NSW, Australia

Extensive surface profiles of colorectal cancer (CRC) cells and tumour infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies.  Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.